Scalable crossflow separation technology
Scalable hollow-fiber and cassette solutions for microfiltration, ultrafiltration and diafiltration.
Nominal pore size or MWCO alone does not guarantee process performance
Module formats
Tangential flow moves process fluid parallel to the membrane while pressure drives smaller components into the permeate. Retained cells, particles or macromolecules remain in the retentate stream for recirculation, concentration or diafiltration.
Modified-PES hollow fibers provide smooth tubular flow paths for cell- or particle-containing feeds, selected perfusion duties and clarification applications.
Separation regime Microfiltration
Modified-PES hollow-fiber ultrafilters combine relatively low hold-up volume with open flow paths for shear-sensitive products and compatible biological assemblies.
Separation regime Ultrafiltration / diafiltration
Regenerated-cellulose and PES cassettes provide broad MWCO choice with tight, coarse, high-viscosity, suspended/open and custom channels.
Channel options Tight to open / custom
Separation rating and flow-path selection
Micrometer pore ratings and molecular-weight cutoffs describe different separation regimes. Membrane chemistry, effective target size, feed solids, viscosity, crossflow, transmembrane pressure and concentration polarization all influence observed retention and flux.
End-user applications
Performance depends on feed composition, viscosity, crossflow, transmembrane pressure, temperature, process time, hold-up volume and channel geometry. Representative trials are required before scale-up.
Cell-culture and fermentation clarification, cell retention and selected perfusion-process operations.
Concentration of monoclonal antibodies, recombinant proteins, enzymes and peptides.
Concentration and purification support for compatible vaccines, viral vectors and biological assemblies.
Concentration of nucleic acids and other compatible high-molecular-weight products.
Desalting, buffer exchange and removal of low-molecular-weight impurities from retained products.
Volume reduction before chromatography or formulation, from laboratory screening through manufacturing.
System-selection guidance
Screen candidate membranes and channels with representative feed, then scale using normalized membrane area, crossflow, transmembrane pressure, system geometry and an appropriate process safety factor.
Define microfiltration, ultrafiltration, concentration or diafiltration duty and whether the target stays in retentate or permeate.
Provide molecular or particle size, aggregation state, solids, turbidity, viscosity and concentration profile.
Select membrane chemistry, pore rating or MWCO, hollow-fiber lumen or cassette screen for the expected feed behavior.
Establish crossflow, shear sensitivity, channel pressure drop, transmembrane pressure and flux targets.
Confirm starting and final volume, concentration factor, diafiltration volume, hold-up volume and recovery target.
Define membrane area, process time, connections, holder, tubing, pump, instrumentation and single-use or reusable approach.
Configuration matrix
Offered combinations are format dependent. Hollow-fiber modules use integral module connections, while cassette devices require a compatible holder and manifold. Confirm product binding, compatibility, cleaning or sanitization exposure, extractables and recovery with the actual process fluid.
| Media | General characteristics | Suggested applications |
|---|---|---|
| Modified PES | Hydrophilic polyethersulfone membrane offering high flow, mechanical strength and broad use across microfiltration and ultrafiltration | Hollow-fiber MF and UF; selected cassette concentration and diafiltration applications |
| Regenerated cellulose | Hydrophilic membrane with low nonspecific protein interaction and strong product-recovery potential | Cassette ultrafiltration of protein-sensitive streams, concentration and buffer exchange |
| Format / channel | Media | Nominal rating | Filtration area | Process scale | Interface |
|---|---|---|---|---|---|
| Hollow-fiber microfiltration | Modified PES | 0.1 / 0.2 / 0.45 / 0.65 µm | 28 / 56 / 118 / 236 / 471 cm²; 0.15–16.8 m² | 300 mL–4 L; 6–1500 L | Female/male Luer; 3/8 in. hose barb; 1/2, 1-1/2 and 2 in. sanitary clamp |
| Hollow-fiber ultrafiltration | Modified PES | 50 / 100 / 300 / 500 / 750 kDa | 28 / 56 / 118 / 236 / 471 cm²; 0.15–16.8 m² | 300 mL–4 L; 6–1500 L | Female/male Luer; 3/8 in. hose barb; 1/2, 1-1/2 and 2 in. sanitary clamp |
| Cassette · Tight screen | RC / PES | 1 / 3 / 5 / 8 / 10 / 30 / 50 / 100 / 300 / 500 / 1000 kDa | 100 cm² | 200–2000 mL | Compatible holder / manifold |
| Cassette · Coarse screen | RC / PES | 1 / 3 / 5 / 8 / 10 / 30 / 50 / 100 / 300 / 500 / 1000 kDa | 200 cm² | 200–2000 mL | Compatible holder / manifold |
| Cassette · Open / high-viscosity screen | RC / PES | 1 / 3 / 5 / 8 / 10 / 30 / 50 / 100 / 300 / 500 / 1000 kDa | 0.11 m² | 5–50 L | Compatible holder / manifold |
| Cassette · Suspended / open channel | RC / PES | 1 / 3 / 5 / 8 / 10 / 30 / 50 / 100 / 300 / 500 / 1000 kDa | 0.46 m² | ≥50 L | Compatible holder / manifold |
| Cassette · Custom screen | RC / PES | 1 / 3 / 5 / 8 / 10 / 30 / 50 / 100 / 300 / 500 / 1000 kDa | 0.5 m² | Not specified | Compatible holder / manifold |
Confirm the separation objective, target location, representative feed, membrane, rating, channel, crossflow, transmembrane pressure, starting and final volumes, recovery, membrane area, system interfaces and lifecycle strategy before submitting the final RFQ for technical review.