Fine microbial-contaminant control
Fine membrane filtration for contamination-risk control in cell-culture processes.
A 0.1 µm pore rating alone does not establish a validated mycoplasma-removal claim
Filter formats
The portfolio supports filtration steps where control of very small microbial contaminants is required. Disc, capsule and cartridge formats cover laboratory evaluation, process development, media preparation and manufacturing-scale operation, with single-use fluid paths or reusable-housing options.
Disc-format filters support small-volume testing, media filterability studies and comparison of single-layer and graded configurations.
Filtration area 10–20 cm²
Ready-to-connect capsules support media preparation and other contamination-controlled process steps from development through manufacturing.
Available scale Development to production
Cartridges support production volumes in compatible reusable housings and allow selection of end adapters and O-ring materials.
Housing interfaces DOE · 222 · 226
Membrane and graded-structure selection
PES and PVDF hydrophilic membranes are available with a 0.1 µm final layer. Single-layer and graded structures allow retention requirements to be balanced with the capacity needed for clean or more complex cell-culture fluids.
End-user applications
Capacity and recovery can be strongly affected by media composition, supplements, colloids and protein content. Representative-fluid testing is essential before scale-up.
Fine filtration of compatible chemically defined culture media.
Filtration of serum-containing or supplement-containing media after capacity and recovery assessment.
Filtration of media components and selected compatible biological raw materials.
Contamination-risk control before sensitive fermentation and cell-culture operations.
Filtration of compatible serum, protein and biological solutions.
Selected intermediate or final steps where product-specific challenge validation supports the required claim.
Filter-selection guidance
Use representative media in small-area studies and challenge the selected configuration at defined operating limits. Scale-up should preserve membrane type, construction and process conditions whenever possible.
Define the required mycoplasma-retention claim and challenge-validation approach.
Provide supplement level, colloidal load, particle content and other fouling contributors.
Assess protein or growth-factor adsorption risk and the required product recovery.
Confirm batch volume, processing-time target, differential pressure and temperature.
Define the integrity-test method, acceptance criteria and sterilization method.
Confirm single-use or reusable configuration and whether an upstream prefilter or graded membrane is required.
Configuration matrix
Availability depends on the selected medium, pore structure, filtration area and filter format. Not every option is available in every combination. Media selection should consider growth-factor and protein adsorption, chemical compatibility, sterilization conditions and the intended integrity-test method.
| Media | General characteristics | Suggested applications |
|---|---|---|
| PES | Hydrophilic membrane offering high liquid flow, easy wetting and low nonspecific adsorption | Cell-culture media, media supplements, buffers and protein-containing biological solutions |
| PVDF | Hydrophilic membrane with low protein adsorption and good mechanical and chemical resistance | Product-recovery-sensitive media, biological solutions and valuable process intermediates |
| Format | Filtration area | Connection or end options |
|---|---|---|
| Disc-format filter | 10–20 cm² | 1/4–1/2 in. stepped hose barb |
| Capsule filter | 180–660 cm²; 0.25–3.2 m²; selected 0.8–2.4 m² configurations | 9/16, 3/4, 1-1/4 and 1-1/2 in. sanitary flanges; 3/8, 1/2, 9/16, 5/8, 3/4 and 1 in. stepped hose barbs; 1/4–1/2 and 1/4–3/8 in. multi-stepped hose barbs; selected 1-1/2 in. sanitary T-style connection |
| Cartridge filter | 180–660 cm²; 0.25–3.2 m² | Double open end, 222 and 226 end configurations; customizable O-ring options |
Confirm the required claim, representative media, membrane, pore structure, filtration area, filter format, integrity-test method, sterilization strategy, connections and challenge-validation package before submitting the final RFQ for technical review.