Assay-development membrane components
Capture membranes, blotting media and absorbent components for lateral-flow, flow-through and immunodetection assay development.
Assay-specific screening before final material approval
Material formats
Immunodiagnostic membranes provide a porous reaction surface that transports the sample and immobilizes capture molecules. In lateral-flow assays, nitrocellulose membrane capillary behavior, surface chemistry and protein binding influence signal development, sensitivity, background and test time. Backing can improve handling and reel-to-reel conversion.
PVDF membranes are commonly used for western and immunoblot transfer, while cellulose, glass-fiber and synthetic pads manage sample acceptance, reagent release and final absorption. Material selection requires empirical testing with the intended sample, reagent system, blocking chemistry and device geometry.
Nitrocellulose provides capillary transport and protein-binding surfaces for lateral-flow and flow-through immunoassay formats.
Primary role Capture and signal development
PVDF membranes support western and immunodetection workflows where immobilized proteins are probed after transfer.
Primary role Protein transfer and detection
Cellulose, glass-fiber and synthetic materials provide controlled sample acceptance, conjugate release and terminal fluid absorption.
Primary role Fluid and reagent management
Assay architecture selection
Flow direction, residence time, binding behavior, signal chemistry and device geometry must be considered together. Material grade is a starting point; performance is established only with the intended sample, reagents and assembled test configuration.
Diagnostic applications
Final sensitivity, background, signal intensity and test time depend on the complete combination of membrane, pads, reagents, sample and housing.
Antigen, antibody, protein and other analyte detection using capillary-flow diagnostic membranes.
Assay development requiring controlled wicking, strong capture and careful background management.
Vertical-flow, dot-blot and line-immunoassay formats using suitable nitrocellulose reaction surfaces.
Immunodetection of proteins transferred to PVDF blotting membranes.
Sample introduction, conjugate release and terminal absorbent-pad construction for integrated rapid-test devices.
Component screening, prototype optimization and transfer to converted roll, sheet or strip formats.
Quotation requirements
Provide complete assay context so flow class, binding behavior, absorbency, backing and material format can be reviewed together.
State lateral-flow, flow-through, blotting or pad function and the target analyte.
Provide sample matrix, conjugate, capture chemistry, blockers and required sensitivity.
Define target test time, capillary-flow class, signal intensity and background limit.
Specify backed or unbacked material, roll or sheet dimensions, strip width and cutting needs.
Define absorbency, reagent release, lot consistency, packaging and documentation requirements.
Confirm performance with intended samples and reagents before approving conversion, storage and final supply criteria.
Configuration matrix
Offered combinations are material- and format-specific. Confirm assay response, conversion fit, storage stability and lot controls using representative components.
| Product type | Media | Membrane format | Flow / wicking rate | Backing | Binding target |
|---|---|---|---|---|---|
| Lateral Flow Diagnostic Membrane | NC / nitrocellulose | Roll / sheet / strip | Fast / medium / slow capillary flow | Backed | Antigen / antibody / protein |
| High Sensitivity Diagnostic Membrane | NC / nitrocellulose | Roll / sheet | Medium to slow flow | Backed or unbacked | Antibody / antigen / capture protein |
| Flow-through Assay Membrane | NC / nitrocellulose | Disc / sheet | Vertical flow, not lateral wicking | Unbacked | Protein / antibody / antigen |
| Western / Immunodetection Membrane | PVDF | Sheet / roll / pre-cut membrane | Not defined by capillary flow | Unsupported or supported by format | Protein binding |
| Absorbent / Sample Pad Material | Cellulose / glass fiber / synthetic fiber | Pad / roll / sheet | Controlled absorbency | Unbacked | Sample transport / reagent release |
NC means nitrocellulose and PVDF means polyvinylidene fluoride. Flow class, backing, material format and conversion dimensions are configuration-specific.
Confirm assay architecture, target analyte, sample matrix, reagents, required sensitivity, flow class, signal and background targets, media, backing, roll or sheet dimensions, strip width, pad function, conversion needs, packaging and documentation before submitting the final RFQ for technical review.