Single-use bioreactor assemblies
Preassembled cultivation chambers that bring mixing, gas delivery, exhaust management, sensing, feeding, sampling and harvest into one disposable fluid path.
Scale and configuration require confirmation with the selected vessel, drive, controller and gas-delivery combination
Product overview
Stirred bioreactor bags install in a compatible single-use vessel and drive system. Integrated process lines and sensor interfaces reduce the number of field-made connections required at installation.
The cultivation chamber brings circulation, gas delivery, exhaust management and process interfaces into one disposable assembly.
Fluid-path approach One disposable assembly
The 50 L format supports development and seed applications, while the 200–2,000 L designs use geometrically similar production-scale proportions.
Scale coverage Development through production
Scale and process selection
Scale selection should consider oxygen demand, gas-flow strategy, agitation, cell density, shear sensitivity and the required working-volume range.
End-user applications
The portfolio supports suspension, adherent, batch, fed-batch and perfusion-ready process strategies where supported by the selected process and external equipment.
Suspension culture for recombinant proteins, antibodies and vaccines.
Viral-vector and gene-therapy production.
Cell-therapy process development and manufacturing.
Adherent or microcarrier-based culture where supported by the process.
Batch and fed-batch cultivation.
High-density or perfusion-ready operation with an external retention device.
Quotation preparation
Provide the culture mode, operating range, gas strategy, sensor approach, process lines and equipment interfaces required for technical selection.
Define organism or cell type, suspension/adherent mode, batch strategy and target density.
State minimum inoculation volume, normal working volume and maximum operating volume.
Provide oxygen-transfer, carbon-dioxide stripping and headspace-gas requirements.
Select electrochemical or optical pH/DO approach, temperature and pressure monitoring.
List inoculation, media, feeds, base, antifoam, sampling, harvest, drain, perfusion and exhaust functions.
Identify the vessel, drive, control platform and required mechanical interfaces.
Technical specification matrix
Bioreactor performance depends on the complete bag, vessel, drive, controller and gas-delivery combination. Process limits and scale-up settings require confirmation for the selected hardware.
| Nominal size | Minimum working volume | Maximum working volume | Approximate turndown |
|---|---|---|---|
| 50 L | 22 L | 50 L | 2.2:1 |
| 200 L | 40 L | 200 L | 5:1 |
| 500 L | 100 L | 500 L | 5:1 |
| 1,000 L | 200 L | 1,000 L | 5:1 |
| 2,000 L | 400 L | 2,000 L | 5:1 |
| System function | Configuration choices |
|---|---|
| Mixing | Bottom-mounted impeller with approximately 40° blade angle; bag geometry and impeller sized to the vessel |
| Gas delivery | Fine sparger around 20 µm plus macro sparger around 0.5 or 1 mm; distribution selected by scale and process |
| Liquid addition | Independent base, feed, antifoam and media lines; inoculation connection available |
| Sampling and harvest | Closed sample line, drain/harvest line and configurable end connections |
| Monitoring | Thermowell; pH and dissolved-oxygen probe ports; optional single-use pressure sensor |
| Exhaust | Primary exhaust-filter assembly with optional backup-filter connection |
| Perfusion readiness | Aseptic connection for external cell-retention or recirculation equipment |
| Tubing and interfaces | Platinum-cured silicone and weldable thermoplastic elastomer; sanitary, quick, luer or aseptic connections |
Confirm the cell line and cultivation mode, working-volume range, gas strategy, sensors, process lines and the selected vessel, drive and controller before technical review.