Laboratory concentration and buffer exchange
Disposable centrifugal ultrafiltration devices for concentrating biomolecules, exchanging buffers and recovering filtrate from microliter- to tens-of-milliliter samples.
MWCO performance and the approved membrane-volume combination must be confirmed for the target molecule and operating conditions
Basic information
Centrifugal filter devices combine a membrane insert with a filtrate receiver. Centrifugal force drives solvent and species below the effective membrane cut-off into the receiver while the target macromolecule remains in the retentate chamber. The format supports rapid concentration without a separate pump or pressure vessel.
A compact disposable format for routine concentration, desalting, buffer exchange and filtrate recovery.
Sample-volume range 0.5-20 mL
A higher-capacity format for concentrating larger laboratory samples while retaining the convenience of centrifugal operation.
Sample-volume range 15-60 mL
Membrane and device selection
Device selection should consider starting volume, desired final volume, target molecular size, membrane chemistry, protein binding, rotor type, maximum centrifugal force and recovery method. RC and PES cover common laboratory concentration duties, but not every volume and MWCO combination is available.
End-user applications
Match the membrane, MWCO, volume and centrifuge conditions to the target molecule, sample matrix and downstream method.
Concentration of proteins, antibodies, enzymes, nucleic acids and viral particles.
Removal of salts, small-molecule reagents and unwanted buffer components.
Preparation before electrophoresis, chromatography or mass spectrometry.
Recovery of low-molecular-weight filtrate for downstream analysis.
Concentration of cell-culture supernatants, lysates and clarified fermentation samples.
Small-volume screening and method development before process scale-up.
Quotation preparation
A complete RFQ should identify the molecule, membrane requirement, working volume, recovery objective and equipment constraints for technical review.
Provide the sample matrix, target molecule, approximate molecular weight and concentration.
State the starting volume, minimum working volume and required final retentate volume.
Select RC or PES based on binding, solvent compatibility and recovery requirements.
Identify rotor type, tube format, available centrifugal force and operating temperature.
Confirm pack size, sterility status, individual packaging and documentation needs.
Configuration matrix
Configuration note. MWCO performance is molecule- and condition-dependent. Confirm rotor compatibility, maximum centrifugal force, minimum retentate volume and the approved membrane-volume combination before use.
| Product type | Sample volume | Media | MWCO |
|---|---|---|---|
| Centrifugal Filter Device | 0.5-20 mL | RC / PES | 3 / 10 / 30 / 50 / 100 / 300 kDa |
| Large-volume Centrifugal Device | 15-60 mL | RC / PES by device | Selected 3 / 10 / 30 / 50 / 100 / 300 kDa; availability varies by volume |
Confirm the sample, target molecule, starting and final volume, membrane, MWCO, rotor conditions and supply requirements before submitting the final RFQ for technical review.